Part:BBa_K3467003:Experience

In our project, we designed three basic part, targeting to three different genes of locust. In our design, we selected Locust migratoria gene trypsin-like serine protease, chitin synthase and ATPase subunitH gene as our target gene. By using specific program to design dsRNA sequence, we finally designed our three basic parts: TSP dsRNA sequence, CHS dsRNA sequence and ATPase dsRNA sequence.

We also measured their effect to locust by injection experiment.

As the result shows:

Fig. 1 CHS dsRNA injection experiment

Comparing with the WT and control group (by injecting DEPC water) locust survival rate, we used LT50 to measure dsRNA effect. From the diagram above, CHS dsRNA injection has a great effect to locust which CHS experiment group LT50 is approximately 4days.

Fig. 2 LT50 of locust dsRNA injection experiment

Fig. 2 is the survival rate that we measure during the whole experiment process.